Human Angiotensin Ⅱ (Ang-Ⅱ) EL

产品描述：Human Angiotensin Ⅱ (Ang-Ⅱ) ELISA Kit

产品别名：Angiotensin-2

应用笔记：This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang-Ⅱ. During the reaction, Human Ang-Ⅱ in the sample or standard competes with a fixed amount of Human Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

实验时长：1.5H

UniProt ID:P01019

靶点：AngII

Note：For research use only.