- ￥3510
- biorbyt
- 156.25-10000pg/mL
- 49.3 pg/mL
- orb1806555
- 英国
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
156.25-10000pg/mL
- 检测方法：
Competitive
- 适应物种：
All
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
49.3 pg/mL
- 规格：
48 T
产品别名：InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol
应用笔记：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Inositol Triphosphate(IP3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inositol Triphosphate(IP3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inositol Triphosphate(IP3) in the samples is then determined by comparing the OD of the samples to the standard curve.
实验时长：1.5H
靶点：IP3
Note：For research use only.
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文献和实验Activation of MAPKs by G Protein-Coupled Receptors
, thereby activating or inhibiting the production of a variety of second messengers, such as cyclic adenosine monophosphate, cyclic guanosine monophosphate, diacylglycerol, inositol triphosphate (IP3 ), and phosphatidyinositol triphosphate (PIP3 ), arachidonic
些含氮激素的作用信息并不以cAMP为媒介进行传递，如胰岛素、催产素、催乳素、某些下丘脑调节肽和生长因子等。实验证明，这些激素作用于膜受体后，往往引起细胞膜磷脂酰肌醇转变成为三磷酸肌醇(inositol-1,4,5,triphosphate,IP3 )和二酰甘油(diacylglycerol,DG)，并导致胞浆中Ca2+ 浓度增高。近年来，有人提出IP3 和DG可能是第二信使的学说引起人们的重视，并且得到越来越我的实验证实。这一学说认为，在激素的作用下，可能通过G蛋白的介导，激活细胞膜内的磷脂
【申请上传】Current Protocols in Immunology
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