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- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 检测范围:
0.16-10 µg/mL
- 检测方法:
Sandwich
- 适应物种:
Equine
- 样本:
Serum, plasma and other biological fluids
- 灵敏度:
0.053 µg/mL
- 规格:
48 T/96 T
| 规格: | 48 T | 产品价格: | ¥3861.0 |
|---|---|---|---|
| 规格: | 96 T | 产品价格: | ¥5499.0 |
描述: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
别名: GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
浓度: 10 µg/mL
应用说明: standard: 10 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse ADP in the samples is then determined by comparing the OD of the samples to the standard curve
检测时间: 3.5h
研究领域: Metabolism Research
备注: For research use only.

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