相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.14 ng/mL
- 规格:
48 T
产品别名:RXRB; NR2B2; Retinoic acid receptor RXR-beta; Nuclear receptor subfamily 2 group B member 2; Retinoid X receptor beta
应用笔记:standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RXRβ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RXRβ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RXRβ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RXRβ in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:3.5h
Note:For research use only.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验FA Extraction of [insoluble] Amyloid beta from Mouse Brain
at room temperature. 5. CAUTION: Sodium azide (NaN3 ) is highly toxic. 实验步骤 1. Mix 200 μl 10% (w/v) homogenate into 440 μl cold formic acid (minimum 95%, Sigma, F-0507) in a microcentrifuge tube. 2. Sonicate
Targeted Somatic Mutagenesis in the Mouse Epidermis
2 , whose activity is induced by antiestrogens such as Tamoxifen (Tam), and which is obtained by fusing the Cre recombinase with a mutated ligand binding domain of the human estrogen receptor ERα. As an example, we present ablation of the retinoid receptor RXRα
Preparation of Polyclonal Antibodies to Retinoid Receptors
of a retinoid “X” receptor α (RXRα) antibody on Western blots contributed to the identification of the RXR ligand by detection of the receptor in fractions of whole-cell lysates that were able to bind the 9-cis retinoic acid ligand (2 ) Antibodies










