- ￥3861 - 5499
- Biorbyt
- 0.16-10 ng/mL
- 0.067 ng/mL
- orb1736791
- 英国
- 2026年08月04日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
Biorbyt
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.067 ng/mL
- 规格：
48 T/96 T
| 规格： | 48 T | 产品价格： | ￥3861.0 |
|---|---|---|---|
| 规格： | 96 T | 产品价格： | ￥5499.0 |
描述: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SELENOF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SELENOF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SELENOF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SELENOF in the samples is then determined by comparing the OD of the samples to the standard curve.
别名: SEP15; 15 kDa selenoprotein
浓度: 10 ng/mL
应用说明: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SELENOF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SELENOF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SELENOF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SELENOF in the samples is then determined by comparing the OD of the samples to the standard curve
检测时间: 3.5h
研究领域: Cell Biology
备注: For research use only.
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