- ￥3900
- biorbyt
- 15.63-1000 pg/mL
- 6.45 pg/mL
- orb1736590
- 英国
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
15.63-1000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Bovine
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
6.45 pg/mL
- 规格：
48 T
产品别名：CXCL6; SCYB6; GCP2; CKA-3; Chemokine(C-X-C-Motif)ligand 6; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 6; Chemokine alpha 3; Small-inducible cytokine B6
应用笔记：standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CXCL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CXCL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CXCL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CXCL6 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:P80221
Note：For research use only.
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文献和实验V294.PART II，Chapter 5 细胞粘附实验（英文版）
by centrifugation in a microfuge at 3800g for 15 min and then keepthe cell pellets in –20°C freezer. Simultaneous to step 7, add 600 μL of the substratesolution to the microtube, vortex, and then transfer the cell/substrate mixtureto new wells of the 96-well ELISA
【申请上传】Current Protocols in Immunology
, Growth Factor, and Chemokine Ligand Database Chapter 7 Immunologic Studies in Humans Introduction Section I Preparation of Human Mononuclear Cell Populations and Subpopulations Unit 7.1 Isolation of Whole Mononuclear Cells from Peripheral Blood
【分享】Human press METHODS IN MOLECULAR BIOLOGY
and Protocols, 1st Ed., Edited by L.C. Javios, 1994 35. Peptide Synthesis Protocols, Edited by Penning- ton, Michael W., 1994 36. Peptide Analysis Protocols Edited by Dunn, Ben M., 1994 37. In Vitro Transcription and Translation Protoc- ols Edited
