Mouse Lipase A, Lysosomal Acid

类别: Assays and Kits

描述: Mouse Lipase A, Lysosomal Acid (LIPA) ELISA Kit

别名: Lysosomal acid lipase/cholesteryl ester hydrolase, Acid cholesteryl ester hydrolase, LAL, Cholesteryl esterase, Lipase A, Sterol esterase, Lipa, Lip1

靶点: LIPA

应用说明: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LIPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LIPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LIPA. You can calculate the concentration of Mouse LIPA in the samples by comparing the OD of the samples to the standard curve.

检测时间: 3.5H

标准品: 10ng/mL

研究领域: Metabolism Research

UniProt ID: Q9Z0M5

备注: For research use only.