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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.16-10ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
0.1 ng/mL
- 规格:
48 T
产品别名:Lysosomal acid lipase/cholesteryl ester hydrolase, Acid cholesteryl ester hydrolase, LAL, Cholesteryl esterase, Lipase A, Sterol esterase, Lipa, Lip1
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LIPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LIPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LIPA. You can calculate the concentration of Mouse LIPA in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:Q9Z0M5
靶点:LIPA
Note:For research use only.
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文献和实验【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
, α-MF[172]Mouse lysosomal acid a-mannosidaseS, native[164]Murine Golgi mannosidase IAS, PHO1[171]Mouse P-glycoprotein mdr3I, active570Mouse Apha-methylacyl-CoA racemaseI, active375Mouse 5HT5A 5-tryptamine receptorS*, 40 pmol/mg,α-MF[161]Mouse salivary
secondary antibody review -- data from 99 publications
epithelial Caco2/bbe cells Bio-Rad 10 rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars
【分享】Human press METHODS IN MOLECULAR BIOLOGY
.humanapress.com 1. Proteins. Edited by Walker, John M., 1984 2. Nucleic Acids. Edited by Walker, John M., 1984 3. New Protein Techniques. Edited by Walker, John M., 1988 4. New Nucleic Acid Techniques. Edited by Walker, John M., 1988 5. Animal Cell












