- ￥3190 - 4301
- Biorbyt
- 62.5-4000 pg/mL
- orb1736452
- 英国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
Biorbyt
- 检测范围：
62.5-4000 pg/mL
- 应用：
详询
- 适应物种：
Human
- 样本：
Serum, plasma, tissue homogenates and other biological fluids.
- 规格：
48 t/96 t
| 规格： | 48 t | 产品价格： | ￥3190.0 |
|---|---|---|---|
| 规格： | 96 t | 产品价格： | ￥4301.0 |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDE4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDE4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDE4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDE4A in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名：Human PDE4A(Phosphodiesterase 4A, cAMP Specific) ELISA Kit
货号：orb1736452
产品中文名：Human PDE4A(Phosphodiesterase 4A, cAMP Specific) ELISA Kit 酶联免疫试剂盒
产品详情链接：https://www.biorbyt.com/human-pde4aphosphodiesterase-orb1736452.html
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文献和实验竞争ELISA检测试剂盒，无需对样品中的cAMP进行乙酰化。 http://www.neweastbio.com/NonAcetylatedCamp.do 小猪亨亨 http://www.mediomics.com/camp_desinger.html 我准备试试这个 neweastbio 如何定量检测cAMP和cGMP
Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion
【求助】如何提取细胞中的cAMP？和普通蛋白的裂解buffer一样么？
试剂盒出售，不是基于ELISA法的，比ELISA法更方便。 苏叶 我用的是125I的RIA kit，我们核同位素室的老师说，照常处理你的细胞，离心去上清后加入0.4ml 1M的高氯酸就可以了。不过要预实验一下，摸索浓度。 我这几天也在做细胞内cAMP和cGMP检测。 sosorunning 普通的Lysis buffer 就可以，但是你得加IBMX抑制PDE的活性。不知道你是想做western还是干吗？ 如果你是要测
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