Maxi CF405M Antibody Labeling

Labeling Tubulin and Quantifying Labeling Stoichiometry

) BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X stock and stored at 4¡C) High pH Cushion : 0.1 M NaHEPES, pH 8.6, 1 mM MgCl2, 1 mM EGTA, 60% (v/v) glycerol Labeling Buffer : 0.1M NaHEPES, pH 8.6, 1 mM

Genomic DNA Labeling Protocol

  Developed for microarray-based comparative genomic hybridization. Genomic DNA can be labeled with a simple random-priming protocol based on Gibco/BRL's Bioprime DNA Labeling kit, though nick translation protocols work too. I routinely

Revised amino-allyl labeling protocol

7.0 using pH paper.   ***Altering the ratio of aminoallyl-dUTP to dTTP will affect the incorporation of Cy dye.     1X dNTP final concentration during labeling   500 m M each dATP, dCTP, dGTP