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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 应用:
详询
- 规格:
20 x 20 reactions
Preparation of randomly IR750-modified RNA probes by in vitro transcription with UTP-PEG5-IR750
产品名:HighYield T7 IR750 RNA Labeling Kit
货号:orb1733303
产品中文名:HighYield T7 IR750 RNA Labeling Kit 试剂盒
产品详情链接:https://www.biorbyt.com/highyield-t7-ir750-rna-labelin-orb1733303.html
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文献和实验(as part of the BioPrime labeling kit), produces better labeling. 6. Incubate 37 degrees C for 1 to 2 hours, then stop reaction by adding 5 ul 0.5 M EDTA pH8.0 7. As with RNA probes, I purify the DNA probe using a microcon 30 filter (Amicon/Millipore
Revised amino-allyl labeling protocol
7.0 using pH paper. ***Altering the ratio of aminoallyl-dUTP to dTTP will affect the incorporation of Cy dye. 1X dNTP final concentration during labeling 500 m M each dATP, dCTP, dGTP
需准备的材料及试剂:1、DEPC水处理的EP管(1.5ml,1.0ml),Tip头 2、玻璃平皿、镊子180℃烘烤6小时,以祛除RNA酶 3、硝酸纤维素膜 4、DigRNA labeling kit(sp6/T7) 5、经分离纯化的线形模板DNA 6、DEPC水处理过的无RNase 的0.2M EDTA,PH=8.0 7、RNA稀释用Buffer 8、BufferⅠ 9、BufferⅡ 10、50×blocking 11、Detection 12、Dig 抗体及显色
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