HHLA2 Antibody (N-term) 抗体,orb1938823,Biorbyt产品图

HHLA2 Antibody (N-term) 抗体,orb

1938823,Biorbyt
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  • ¥2106 - 3861
  • Biorbyt已认证
  • orb1938823
  • 英国
  • 2026年08月07日
  • FC, IHC-P, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      HHLA2 Antibody (N-term) 抗体

    • 抗体英文名

      HHLA2 Antibody (N-term)

    • 靶点

      HHLA2

    • 应用范围

      FC, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1938823

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型

      Rabbit IgG

    • 免疫原

      详询

    • 规格

      50 ul/100 ul

    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3861.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Affinity Purified Rabbit Polyclonal Antibody (Pab)

    别名: HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2, HHLA2

    应用稀释比例: WB - 1:2000, IHC-P - 1:25, FC - 1:25

    免疫原: This HHLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids of human HHLA2. Antigen Region: 58-87 aa.

    分子量: 46850 Da

    研究领域: Cell Biology, Immunology & Inflammation

    UniProt ID: Q9UM44

    NCBI 参考序列: NP_009003.1

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    All lanes: Anti-HHLA2 Antibody (N-term) at 1:2000 dilution. Lane 1: Human kidney lysate. Lane 2: A549 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片2
    All lanes: Anti-HHLA2 Antibody (N-term) at 1:2000 dilution. Lane 1: Human kidney lysate. Lane 2: Human lung lysate. Lane 3: Human pancreas lysate. Lane 4: Human testis lysate. Lane 5: MDA-MB-453 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片3
    All lanes: Anti-HHLA2 Antibody (N-term) at 1:2000 dilution. Lane 1: Human kidney lysate. Lane 2: Human lung lysate. Lane 3: Human testis lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片4
    Anti-HHLA2 Antibody (N-term) at 1:2000 dilution + Human kidney tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片5
    Staining HHLA2 in human kidney tissue sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

    产品细节图片6
    Staining HHLA2 in human pancreas tissue sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

    产品细节图片7
    HHLA2 Antibody (N-term) (orb1938823) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHLA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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    图标文献和实验
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    • Antibody Storage

      to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain

    • Biotinylation of Antibody

        Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust

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