HDAC9 Antibody (N-term) 抗体,orb1938455,Biorbyt产品图

HDAC9 Antibody (N-term) 抗体,orb

1938455,Biorbyt
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  • ¥4095
  • Biorbyt已认证
  • orb1938455
  • 英国
  • 2026年08月07日
  • IF, IHC-P, IP, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      HDAC9 Antibody (N-term) 抗体

    • 抗体英文名

      HDAC9 Antibody (N-term)

    • 靶点

      HDAC9

    • 应用范围

      IF, IHC-P, IP, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1938455

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

    • 亚型

      Rabbit IgG

    • 免疫原

      详询

    • 规格

      100 ul

    类别: Antibodies

    描述: Purified Rabbit Polyclonal Antibody (Pab)

    别名: Histone deacetylase 9, HD9, Histone deacetylase 7B, HD7, HD7b, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR

    应用稀释比例: IF - 1:1,000, IP - 1:100, WB - 1:1000, IHC-P - 1:50-100

    免疫原: This HDAC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human HDAC9. Antigen Region: 2-32 aa.

    分子量: 111297 Da

    预测反应性: Mouse

    研究领域: Epigenetics & Chromatin

    UniProt ID: Q9UKV0

    NCBI 参考序列: NP_848512.1, NP_848510.1, NP_001191075.1, NP_001191073.1, NP_055522.1, NP_001191076.1, NP_001191077.1, NP_001191074.1, NP_478056.1

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    Both anti-HDAC9 N-term and C-term Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.

    产品细节图片2
    Immunoblots for MITR (HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR.

    产品细节图片3
    Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a FLAG antibody (bottom panel) detecting a FLAG epitope fused at the N-term end of the MITR construct.

    产品细节图片4
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

    产品细节图片5
    This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells.

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    图标文献和实验
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    • Antibody Storage

      to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain

    • Biotinylation of Antibody

        Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust

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