LZIC Antibody (Center) 抗体,orb1933772,Biorbyt产品图

LZIC Antibody (Center) 抗体,orb1

933772,Biorbyt
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  • ¥2106 - 3861
  • Biorbyt已认证
  • orb1933772
  • 英国
  • 2026年08月07日
  • FC, IF, IHC-P, WB
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      LZIC Antibody (Center) 抗体

    • 抗体英文名:

      LZIC Antibody (Center)

    • 靶点:

      LZIC

    • 应用范围:

      FC, IF, IHC-P, WB

    • 宿主:

      Rabbit

    • 适应物种:

      Human, Rat

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb1933772

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 形态:

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型:

      Rabbit IgG

    • 免疫原:

      详询

    • 规格:

      50 ul/100 ul

    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3861.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Affinity Purified Rabbit Polyclonal Antibody (Pab)

    别名: Protein LZIC, Leucine zipper and CTNNBIP1 domain-containing protein, Leucine zipper and ICAT homologous domain-containing protein, LZIC

    应用稀释比例: IF - 1:25, WB - 1:2000, IHC-P-Leica - 1:500, FC - 1:25

    免疫原: This LZIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-109 amino acids from the Central region of human LZIC. Antigen Region: 81-109 aa.

    分子量: 21495 Da

    预测反应性: Mouse, Zebrafish

    研究领域: Neuroscience

    UniProt ID: Q8WZA0

    NCBI 参考序列: NP_115744.2

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    Immunohistochemical analysis of paraffin-embedded human kidney tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

    产品细节图片2
    All lanes: Anti-LZIC Antibody (Center) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片3
    Immunofluorescent analysis of 4% Fixative-fixed, 0.1% Triton X-100 permeabilized Hela cells labeling LZIC at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus and Cytoplasm, Weak Membrane staining on Hela cell line. The nuclear counter stain is DAPI (blue).

    产品细节图片4
    Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

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