LYN Antibody (N-term) 抗体,orb1928953,Biorbyt产品图

LYN Antibody (N-term) 抗体,orb19

28953,Biorbyt
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  • ¥10764
  • Biorbyt已认证
  • orb1928953
  • 英国
  • 2026年08月07日
  • FC, IHC-P, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      LYN Antibody (N-term) 抗体

    • 抗体英文名:

      LYN Antibody (N-term)

    • 靶点:

      LYN

    • 应用范围:

      FC, IHC-P, WB

    • 宿主:

      Rabbit

    • 适应物种:

      Human

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb1928953

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 形态:

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

    • 亚型:

      Rabbit IgG

    • 免疫原:

      详询

    • 规格:

      400 ul

    类别: Antibodies

    描述: Purified Rabbit Polyclonal Antibody (Pab)

    别名: Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, LYN, JTK8

    应用稀释比例: WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50

    免疫原: This LYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-62 amino acids from the N-terminal region of human LYN. Antigen Region: 32-62 aa.

    分子量: 58574 Da

    研究领域: Signal Transduction

    UniProt ID: P07948

    NCBI 参考序列: NP_001104567.1, NP_002341.1

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    Western blot analysis of anti-LYN Antibody (N-term) in HL60 cell line lysates (35 ug/lane). LYN (arrow) was detected using the purified Pab.

    产品细节图片2
    LYN Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

    产品细节图片3
    Anti-LYN Antibody (E46) at 1:1000 dilution + Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片4
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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    图标文献和实验
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    • Antibody Storage

      to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain

    • Biotinylation of Antibody

        Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust

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