BMI1 Antibody 抗体，orb1928300，Biorbyt产品图

BMI1 Antibody 抗体，orb1928300，Bi

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  • ￥2106 - 3861
  • Biorbyt已认证
  • orb1928300
  • 英国
  • 2026年08月07日
  • FC, IF, IHC-P, WB
  • Rabbit
  • Human, Mouse
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      BMI1 Antibody 抗体

    • 抗体英文名：

      BMI1 Antibody

    • 靶点：

      BMI1

    • 应用范围：

      FC, IF, IHC-P, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse

    • 保质期：

      12 months from date of receipt.

    • 抗原来源：

      详询

    • 目录编号：

      orb1928300

    • 级别：

      科研

    • 库存：

      99

    • 供应商：

      Biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      详询

    • 形态：

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型：

      Rabbit IgG

    • 免疫原：

      详询

    • 规格：

      50 ul/100 ul

    规格：50 ul产品价格：￥2106.0
    规格：100 ul产品价格：￥3861.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Affinity Purified Rabbit Polyclonal Antibody (Pab)

    别名: Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51

    应用稀释比例: IF - 1:25, WB - 1:8000, IHC-P - 1:10-50, FC - 1:25

    免疫原: This BMI1 antibody is generated from rabbits immunized with BMI1 recombinant protein.

    分子量: 36949 Da

    研究领域: Epigenetics & Chromatin

    UniProt ID: P35226

    NCBI 参考序列: NP_001190991.1, NP_005171.4

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with BMI1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

    产品细节图片2
    All lanes: Anti-BMI1 Antibody at 1:8000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片3
    All lanes: Anti-BMI1 Antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片4
    Immunofluorescent analysis of 4% Fixative-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling BMI1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

    产品细节图片5
    Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

    产品细节图片6
    Overlay histogram showing A549 cells stained (green line). The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

    产品细节图片7
    Overlay histogram showing U-2 OS cells stained (green line). The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

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