SLC22A2 Antibody (N-term) 抗体,orb1926160,Biorbyt产品图

SLC22A2 Antibody (N-term) 抗体,o

rb1926160,Biorbyt
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  • ¥2106 - 3861
  • Biorbyt已认证
  • orb1926160
  • 英国
  • 2026年08月07日
  • FC, IHC-P, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      SLC22A2 Antibody (N-term) 抗体

    • 抗体英文名

      SLC22A2 Antibody (N-term)

    • 靶点

      SLC22A2 (HGNC:10966)

    • 应用范围

      FC, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1926160

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型

      Rabbit IgG

    • 免疫原

      详询

    • 规格

      50 ul/100 ul

    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3861.0
    类别: Antibodies

    描述: Purified Rabbit Polyclonal Antibody (Pab)

    别名: Solute carrier family 22 member 2, Organic cation transporter 2, hOCT2, SLC22A2, OCT2

    应用稀释比例: WB - 1:2000, IHC-P - 1:25, FC - 1:25

    免疫原: This SLC22A2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 57-89 amino acids from the N-terminal region of human SLC22A2.

    分子量: 62581 Da

    研究领域: Signal Transduction

    UniProt ID: O15244

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    All lanes: Anti-SLC22A2 Antibody (N-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片2
    Staining SLC22A2 in human kidney tissue sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    产品细节图片3
    Staining SLC22A2 in human stomach tissue sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    产品细节图片4
    Overlay histogram showing A431 cells stained (green line). The cells were fixed with 2% Fixative (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

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    图标文献和实验
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    • Antibody Storage

      to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain

    • Biotinylation of Antibody

        Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust

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    SLC22A2 Antibody (N-term) 抗体,orb1926160,Biorbyt
    ¥2106 - 3861