Hsp60 Antibody 抗体，orb1925739，Biorbyt产品图

Hsp60 Antibody 抗体，orb1925739，B

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  • ￥2106 - 3861
  • Biorbyt已认证
  • orb1925739
  • 英国
  • 2026年08月07日
  • IF, IHC-P, WB
  • Rabbit
  • Human, Mouse
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      Hsp60 Antibody 抗体

    • 抗体英文名：

      Hsp60 Antibody

    • 靶点：

      HSPD1

    • 应用范围：

      IF, IHC-P, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse

    • 保质期：

      12 months from date of receipt.

    • 抗原来源：

      详询

    • 目录编号：

      orb1925739

    • 级别：

      科研

    • 库存：

      99

    • 供应商：

      Biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      详询

    • 形态：

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型：

      Rabbit IgG

    • 免疫原：

      详询

    • 规格：

      50 ul/100 ul

    规格：50 ul产品价格：￥2106.0
    规格：100 ul产品价格：￥3861.0
    类别: Antibodies

    描述: Purified Rabbit Polyclonal Antibody (Pab)

    别名: 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60

    应用稀释比例: IF - 1:25, WB - 1:2000, IHC-P - 1:25

    免疫原: This Hsp60 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 340-374 amino acids from the human region of human Hsp60.

    分子量: 61055 Da

    预测反应性: Bovine

    研究领域: Neuroscience

    UniProt ID: P10809

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    All lanes: Anti-Hsp60 Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: mouse thymus lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片2
    Immunofluorescent analysis of 4% Fixative-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Hsp60 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).

    产品细节图片3
    All lanes: Anti-Hsp60 Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: mouse thymus lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片4
    Staining Hsp60 in human kidney tissue sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    产品细节图片5
    Staining Hsp60 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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