PABPN1L Rabbit Polyclonal Antibody 抗体,orb1880735,Biorbyt产品图

PABPN1L Rabbit Polyclonal Anti

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  • ¥1638 - 4329
  • Biorbyt已认证
  • orb1880735
  • 英国
  • 2026年08月06日
  • FC, IF, IHC, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      PABPN1L Rabbit Polyclonal Antibody 抗体

    • 抗体英文名

      PABPN1L Rabbit Polyclonal Antibody

    • 靶点

      PABPN1L

    • 应用范围

      FC, IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1880735

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Liquid

    • 免疫原

      详询

    • 规格

      30 ul/50 ul/100 ul/200 ul

    规格:30 ul产品价格:¥1638.0
    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3159.0
    规格:200 ul产品价格:¥4329.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: The PABPN1L Antibody is suitable for FC, IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human PABPN1L. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名: EPABP2; PABPNL1; Embryonic polyadenylate-binding protein 2; Embryonic poly(A)-binding protein 2; ePABP-2; ePABP2; Embryonic poly(A)-binding protein type II; Poly(A)-binding protein nuclear-like 1

    应用稀释比例: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50), FC (1/10 - 1/50)

    免疫原: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human PABPN1L. The exact sequence is proprietary.

    防腐剂: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    纯化方法: The antibody was purified by immunogen affinity chromatography.

    特异性: Recognizes endogenous levels of PABPN1L protein.

    UniProt ID: A6NDY0

    Entrez 基因 ID: 390748

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of PABPN1L expression in A549 (A), human kidney (B) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 30 kD)

    产品细节图片2
    Immunohistochemical analysis of PABPN1L staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of Anti-PABPN1L staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

    产品细节图片4
    Flow cytometric analysis of A549 cells using Anti-PABPN1L Antibody. The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

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    图标文献和实验
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