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- Jena Bioscience
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- NU-1119L
- 2025年07月16日
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- 文献和实验
- 技术资料
- 保存条件：
store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
- 保质期：
1年
- 英文名：
dPTP
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
173964-83-5
dPTP，dPTP，dPTP,dPTP
产品货号：NU-1119L
产品规格：5x30ul(10mM)
dPTP; Nucleotides & Nucleosides; 【CAS Number】173964-83-5
dPTP; Nucleotides & Nucleosides; 【CAS Number】173964-83-5
dPTP-dPTPstore at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
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文献和实验Preparation of Competent Cells (from PMB)
1.Inoculate a couple of 20 ml LB broth in a 125 ml flasks with 0.5 ml of an overnight culture of the recipient strain. 2.Shake at 37 ℃ until O.D. 600=0.13 - 0.15 (1 - 2 hours). Measure by taking some of the broth out with a sterile DPTP
NH4Ac and EtOH precipitation of DNA
% EtOH rinse (carefully and a little slowly add the EtOH by a DPTP: if the pellet comes loose, spin it again), drain the tube again, and dry the pellet briefly (under the vacuum for 5 to 10 min) before resuspending the DNA in 10:2 TE (almost always TE
(carefully and a little slowly add the EtOH by a DPTP: if the pellet comes loose, spin it again), drain the tube again, and dry the pellet briefly (under the vacuum for 5 to 10min) before resuspending the DNA in 10:2 TE (almost always TE except doing a digest
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