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- 文献和实验
- 技术资料
- 抗体名:
Phospho-Cyclin B1 (Ser126) Rabbit Polyclonal Antibody (BF488) 抗体
- 抗体英文名:
Phospho-Cyclin B1 (Ser126) Rabbit Polyclonal Antibody (BF488)
- 靶点:
CCNB1
- 浓度:
1mg/ml
- 应用范围:
ICC, IF
- 宿主:
Rabbit
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1590325
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
BF488
- 克隆性:
Polyclonal
- 保存条件:
详询
- 亚型:
IgG
- 免疫原:
详询
- 规格:
100 ul
产品子类型: Primary Antibody
描述: Phospho-Cyclin B1 (Ser126) Rabbit Polyclonal Antibody (BF488) is a BF488 conjugated antibody targeting CCNB1. This antibody is suitable for ICC, IF. It exhibits reactivity with Human samples.
别名: Cyclin B1(phospho S126); Cyclin B1(phospho-Ser126); p-Cyclin B1(S126); p-Cyclin B1(Ser126); p-Cyclin B1(phospho S126); CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
应用稀释比例: IF=1:100-500, ICC/IF=1:100-500
免疫原: KLH conjugated synthesised phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser126 TA(p-S)PS
防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化方法: Affinity purified by Protein A
研究领域: Signal Transduction
保存条件: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
备注: For research use only.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
This protocol is for use with the D and E cyclins and employs 488 nm argon laser excitation of propidium iodide and a FITC-conjugated phenotypic label, and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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