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CEACAM5 Mouse Monoclonal Antib

ody抗体,orb1816926,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb1816926
  • 英国
  • 2025年12月04日
  • IF, IHC-Fr, IHC-P, WB
  • Mouse
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      CEACAM5 Mouse Monoclonal Antibody抗体

    • 抗体英文名

      CEACAM5 Mouse Monoclonal Antibody

    • 靶点

      CEACAM5

    • 浓度

      1mg/ml

    • 应用范围

      IF, IHC-Fr, IHC-P, WB

    • 宿主

      Mouse

    • 适应物种

      Human

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb1816926

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Monoclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      Recombinant human CEACAM5 protein

    • 规格

      50 ul

    产品描述:CEACAM5 Mouse Monoclonal Antibody

    别名:Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100.

    免疫原:Recombinant human CEACAM5 protein

    克隆性:3C12

    分子量:180-200 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P06731

    Note:For research use only.

    产品细节图片1
    paraffin embedded (human appendix), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

    产品细节图片2
    Fixative-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

    产品细节图片5
    Fixative-fixed, paraffin embedded (human esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

    产品细节图片6
    Fixative-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

    产品细节图片7
    Fixative-fixed, paraffin embedded (human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

    产品细节图片8
    Sample: Lane 1: Human LOVO cell lysates, Lane 2: Recombinant human CEACAM5 protein, C-His, Primary: Anti-CEACAM5 (orb1816926) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 77 kDa, Observed band size: 200, 140 kDa.

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    图标文献和实验
    相关实验
    • Rapid Isotyping of Mouse Monoclonal Antibodies

      , isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1  Isotype-Dependent Properties of Monoclonal

    • Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies

      Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII

    • Monoclonal Antibody Production

      and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let

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