- ￥3770
- biorbyt
- orb1240088
- 英国
- 2025年12月08日
- ELISA, IF, IHC-P, WB
- Human
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- 详细信息
- 技术资料
- 抗体名：
TEM1 Antibody抗体
- 抗体英文名：
TEM1 Antibody
- 靶点：
CD248
- 浓度：
1 mg/mL
- 应用范围：
ELISA, IF, IHC-P, WB
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1240088
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
Anti-TEM-1 antibody (orb1240088) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1. The immunogen is located within the last 50 amino acids of TEM-1.
- 规格：
0.02 mg
产品描述：TEM1 Antibody
别名:TEM1 Antibody: TEM1, CD164L1, CD248, Endosialin, Tumor endothelial marker 1
免疫原：Anti-TEM-1 antibody (orb1240088) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1. The 免疫原 is located within the last 50 amino acids of TEM-1.
预测反应性:Mouse, Rat
分子量:Predicted: 81kD and 46kDObserved: 90 kD (post-modifications: 27 O-linked glycosylations) and 46kD
防腐剂:TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.
纯化:TEM1 Antibody is affinity chromatography purified via peptide column.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:NP_065137
UniProt ID:Q9HCU0
Note:For research use only.
别名:TEM1 Antibody: TEM1, CD164L1, CD248, Endosialin, Tumor endothelial marker 1
免疫原：Anti-TEM-1 antibody (orb1240088) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1. The 免疫原 is located within the last 50 amino acids of TEM-1.
预测反应性:Mouse, Rat
分子量:Predicted: 81kD and 46kDObserved: 90 kD (post-modifications: 27 O-linked glycosylations) and 46kD
防腐剂:TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.
纯化:TEM1 Antibody is affinity chromatography purified via peptide column.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:NP_065137
UniProt ID:Q9HCU0
Note:For research use only.
Western Blot Validation in Human Colon Tissue. Loading: 15 µg of lysates per lane. Antibodies: TEM-1 orb1240088, (A, 0.5 µg/mL; B, 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TEM-1 orb1240088, (2 µg/mL), TEM-1 orb1240092, (1 µg/mL), and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TEM-1 orb1240088, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of TEM-1 in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody (orb1240088) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of TEM-1 in Human Colon Tissue. Immunofluorescent analysis of 4% Fixative-fixed Human Colon Tissue labeling TEM-1 with orb1240088 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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TEM1 Antibody抗体，orb1240088，biorbyt
￥3770
