EGFR Antibody 抗体，orb1788107，Biorbyt产品图

EGFR Antibody 抗体，orb1788107，Bi

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  • Biorbyt已认证
  • orb1788107
  • 英国
  • 2026年08月06日
  • FC, IHC, WB
  • Mouse
  • Human
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      EGFR Antibody 抗体

    • 抗体英文名：

      EGFR Antibody

    • 靶点：

      EGFR (HGNC:3236)

    • 应用范围：

      FC, IHC, WB

    • 宿主：

      Mouse

    • 适应物种：

      Human

    • 保质期：

      12 months from date of receipt.

    • 抗原来源：

      详询

    • 目录编号：

      orb1788107

    • 级别：

      科研

    • 库存：

      99

    • 供应商：

      Biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Monoclonal

    • 保存条件：

      详询

    • 形态：

      Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

    • 亚型：

      IgG1κ

    • 免疫原：

      详询

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Purified Mouse Monoclonal Antibody (Mab)

    别名: Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

    应用稀释比例: WB: 1:1000, IHC: 1:25, IHC: 1:25, FC: 1:25

    免疫原: Purified His-tagged EGFR protein(Fragment) was used to produced this monoclonal antibody. Antigen Region: 49-299 aa.

    分子量: 134277 Da

    UniProt ID: P00533

    NCBI 参考序列: NP_958441.1, NP_958439.1, NP_958440.1, NP_005219.2

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    All lanes: Anti-EGFR Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: MDA-MB-468 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 134 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片2
    Staining EGFR in human hepatic carcinoma sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    产品细节图片3
    Staining EGFR in human lung adenocarcinoma sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    产品细节图片4
    Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

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