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文献和实验Null Mutant Mice for Thyroid Hormone Receptors
In mammals, thyroid hormones (TH) have been shown to control the postnatal development of many organs, such as brain, intestine and long bone, and to participate in the maintenance of homeostasis in adults by controlling basal metabolism
This protocol is essentially as described by Murray (1991), Cell Cycle Extracts. In Methods in Cell Biology, B.K. Kay and B. Peng, eds
在开展组织培养技术的初期,为了使所培养的组织能够增殖,认为有必要向培养基中添加胚的生理盐水提取物(胚压榨汁, embryo juice)。对胚提取物的有效成分虽曾进行过许多分离和测定,但并未成功。目前,由于对组织培养基已经有了改良,所以不添加这种成分较为复杂的胚提取物,也能使培养的组织得到充分的增殖,所以现在胚提取物已经很少使用了。
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