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文献和实验Assays for Detection of RNase A Superfamily Ribonucleases
. There are currently six known human members of this superfamily, including pancreatic ribonuclease (RNase 1), eosinophil-derived neurotoxin (RNase 2), eosinophil cationic protein (RNase 3), RNase 4, angiogenin (RNase 5), and RNase 6 (2 ,3 ). Although elevated levels of serum
A, angiogenin, RNase-2 (also known as eosinophil-derived neurotoxin (EDN) or placental RNase), and RNase-4 are inhibited with K i values between 10−14 and 10−16 M . These affinities are among the highest reported for noncovalent binding of proteins. The binding
Embryonic Stem Cells as a Model for Studying Melanocyte Development
Melanocytes residing in the skin, inner ear, and uveal tract are derived from neural crest cells. They are highly dendritic, heavily pigmented, and are generally located in the epidermal basal cell layer of these areas, including hair
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