- ￥1960
- BOSTER
- A05497-4-100ul
- 中国
- 2025年07月14日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A05497-4 |
|---|---|
| 产品名称 | Anti-NEIL3 Antibody |
| 基因名 | NEIL3 |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 70KD |
| 免疫原 | E.coli-derived human NEIL3 recombinant protein (Position: K244-K585). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Nei endonuclease VIII-like 3 (E. coli) is a protein in humans that is encoded by the NEIL3 gene. NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. |
| 研究类别 | 1. Bandaru, V., Sunkara, S., Wallace, S. S., Bond, J. P. A novel human DNA glycosylase that removes oxidative DNA damage and is homologous to Escherichia coli endonuclease VIII. DNA Repair 1: 517-529, 2002.2. Morland, I., Rolseth, V., Luna, L., Rognes, T., Bjoras, M., Seeberg, E. Human DNA glycosylases of the bacterial Fpg/MutM superfamily: an alternative pathway for the repair of 8-oxoguanine and other oxidation products in DNA. Nucleic Acids Res. 30: 4926-4936, 2002. |
| Uniprot ID | NEIL3: Q8TAT5 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Direct ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-NEIL3 antibody (A05497-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NEIL3 antigen affinity purified polyclonal antibody (A05497-4) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NEIL3 at approximately 70 kDa. The expected band size for NEIL3 is at 62-68 kDa.|Figure 2. IHC analysis of NEIL3 using anti-NEIL3 antibody (A05497-4).
NEIL3 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of NEIL3 using anti-NEIL3 antibody (A05497-4).
NEIL3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IHC analysis of NEIL3 using anti-NEIL3 antibody (A05497-4).
NEIL3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.[/list_product_images]
Lane 1: human 293T whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NEIL3 antigen affinity purified polyclonal antibody (A05497-4) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NEIL3 at approximately 70 kDa. The expected band size for NEIL3 is at 62-68 kDa.|Figure 2. IHC analysis of NEIL3 using anti-NEIL3 antibody (A05497-4).
NEIL3 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of NEIL3 using anti-NEIL3 antibody (A05497-4).
NEIL3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IHC analysis of NEIL3 using anti-NEIL3 antibody (A05497-4).
NEIL3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.[/list_product_images]
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Anti-NEIL3 Antibody(A05497-4-100ul)
￥1960
