- ￥1960
- BOSTER
- A08413-3-100ul
- 中国
- 2025年07月12日
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大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A08413-3 |
|---|---|
| 产品名称 | Anti-STAP2 Antibody |
| 基因名 | STAP2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 免疫原 | E.coli-derived human STAP2 recombinant protein (Position: E95-Q392). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Signal-transducing adaptor protein 2 is a protein that in humans is encoded by the STAP2 gene. This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. Minoguchi, M., Minoguchi, S., Aki, D., Joo, A., Yamamoto, T., Yumioka, T., Matsuda, T., Yoshimura, A. STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif. J. Biol. Chem. 278: 11182-11189, 2003.2. Mitchell, P. J., Sara, E. A., Crompton, M. R. A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK. Oncogene 19: 4273-4282, 2000. |
| Uniprot ID | STAP2: Q9UGK3 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- STAP2 antibody (A08413-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human LNCAP whole cell lysates.
Use rabbit anti- STAP2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for STAP2 at approximately 50-55KD. The expected band size for STAP2 is at 45KD.|Figure 2. IHC analysis using anti- STAP2 antibody (A08413-3). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- STAP2 antibody (A08413-3). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human LNCAP whole cell lysates.
Use rabbit anti- STAP2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for STAP2 at approximately 50-55KD. The expected band size for STAP2 is at 45KD.|Figure 2. IHC analysis using anti- STAP2 antibody (A08413-3). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- STAP2 antibody (A08413-3). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-STAP2 Antibody(A08413-3-100ul)
￥1960
