- ￥1960
- BOSTER
- BM4452-100ul
- 中国
- 2025年07月16日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BM4452 |
|---|---|
| 产品名称 | Anti-SGK1 Antibody |
| 基因名 | SGK1 |
| 抗体来源 | Rabbit |
| 克隆 | Monoclonal |
| 抗体亚型 | Rabbit |
| 分子量 | 55KD |
| 免疫原 | A synthesized peptide derived from human SGK1 |
| 内容 | 500 ug/ml;Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 纯化方式 | Affinity-chromatography |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Serine/threonine-protein kinase Sgk1 also known as serum and glucocorticoid-regulated kinase 1 is an enzyme that in humans is encoded by the SGK1 gene. It is mapped to 6q23.2. This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| 研究类别 | 1. Arteaga, M. F., Coric, T., Straub, C., Canessa, C. M. A brain-specific SGK1 splice isoform regulates expression of ASIC1 in neurons. Proc. Nat. Acad. Sci. 105: 4459-4464, 2008. 2. Arteaga, M. F., Wang, L., Ravid, T., Hochstrasser, M., Canessa, C. M. An amphipathic helix targets serum and glucocorticoid-induced kinase 1 to the endoplasmic reticulum-associated ubiquitin-conjugation machinery. Proc. Nat. Acad. Sci. 103: 11178-11183, 2006.3. Gamper, N., Fillon, S., Feng, Y., Friedrich, B., Lang, P. A., Henke, G., Huber, S. M., Kobayashi, T., Cohen, P., Lang, F. K+ channel activation by all three isoforms of serum- and glucocorticoid-dependent protein kinase SGK. Pflugers Arch. 445: 60-66, 2002. |
| Uniprot ID | SGK1: O00141 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:400-800 |
| Immunohistochemistry in paraffin section (IHC): | 1:20-100 |
| Immunocoprecipitation: | 1:20 |
| Flow cytometry (FCM): | 1:20 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
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