- ￥1960
- BOSTER
- A05752-1-100ul
- 中国
- 2025年07月13日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A05752-1 |
|---|---|
| 产品名称 | Anti-PPP1R14A Antibody |
| 基因名 | PPP1R14A |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 20KD |
| 免疫原 | E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126). Human CPI17 alpha shares 83.5% and 84.5% amino acid (aa) sequence identity with mouse and rat CPI17 alpha, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| 研究类别 | 1. Eto M, Ohmori T, Suzuki M, et al. (1996). "A novel protein phosphatase-1 inhibitory protein potentiated by protein kinase C. Isolation from porcine aorta media and characterization.". J. Biochem. 118 (6): 1104–7.2. Yamawaki K, Ito M, Machida H, Moriki N, Okamoto R, Isaka N, Shimpo H, Kohda A, Okumura K, Hartshorne DJ, Nakano T (Jul 2001). "Identification of human CPI-17, an inhibitory phosphoprotein for myosin phosphatase". Biochem Biophys Res Commun. 285 (4): 1040–5. |
| Uniprot ID | PPP1R14A: Q96A00 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| Immunocytochemistry/Immunofluorescence (ICC/IF) | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of CPI17 alpha using anti- CPI17 alpha antibody (A05752-1). Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: PANC-1 whole Cell lysates. anti- CPI17 alpha antigen affinity purified polyclonal antibody (Catalog # A05752-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for CPI17 alpha at approximately 20KD. The expected band size for CPI17 alpha is at 20KD.|Figure 2. IHC analysis of CPI17 alpha using anti- CPI17 alpha antibody (A05752-1).anti- CPI17 alpha Antibody (A05752-1) was detected in paraffin-embedded section of mouse testis tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of CPI17 alpha using anti- CPI17 alpha antibody (A05752-1).anti- CPI17 alpha Antibody (A05752-1) was detected in paraffin-embedded section of rat lung tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of CPI17 alpha using anti- CPI17 alpha antibody (A05752-1).anti- CPI17 alpha Antibody (A05752-1) was detected in paraffin-embedded section of human intestinal cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis of CPI17 alpha using anti- CPI17 alpha antibody (A05752-1).anti- CPI17 alpha Antibody (A05752-1) was detected in paraffin-embedded section of human lung cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis of CPI17 alpha using anti- CPI17 alpha antibody (A05752-1).anti- CPI17 alpha Antibody (A05752-1) was detected in paraffin-embedded section of human placenta tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 7. Flow Cytometry analysis of U20S cells using anti- CPI17 alpha antibody (A05752-1).
Overlay histogram showing U20S cells stained with A05752-1 (Blue line).anti- CPI17 alpha Antibody (A05752-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 7. Immunofluorescence assay analysis of U20S cell at a dilution of 1:50 dylight 488 goat anti-rabbit IgG(green) blue Pseudocolor blue=DAPI.[/list_product_images]
Overlay histogram showing U20S cells stained with A05752-1 (Blue line).anti- CPI17 alpha Antibody (A05752-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 7. Immunofluorescence assay analysis of U20S cell at a dilution of 1:50 dylight 488 goat anti-rabbit IgG(green) blue Pseudocolor blue=DAPI.[/list_product_images]
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Anti-CPI17 alpha/PPP1R14A Antibody(A05752-1-100ul)
￥1960
