- ￥1960
- BOSTER
- A01217-2-100ul
- 中国
- 2025年07月13日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A01217-2 |
|---|---|
| 产品名称 | Anti-S100A4 Antibody |
| 基因名 | S100A4 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 12KD |
| 免疫原 | E.coli-derived human S100A4 recombinant protein (Position: A2-K101). |
| 内容 | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | S100 calcium-binding protein A4 (S100A4) is a protein that in humans is encoded by the S100A4 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| 研究类别 | 1. Ambartsumian, N., Tarabykina, S., Grigorian, M., Tulchinsky, E., Hulgaard, E., Georgiev, G., Lukanidin, E. Characterization of two splice variants of metastasis-associated human mts1 gene. Gene 159: 125-130, 1995. 2. Dorin, J. R., Emslie, E., van Heyningen, V. Related calcium-binding proteins map to the same subregion of chromosome 1q and to an extended region of synteny on mouse chromosome 3. Genomics 8: 420-426, 1990.3. Engelkamp, D., Schafer, B. W., Erne, P., Heizmann, C. W. S100-alpha, CAPL, and CACY: molecular cloning and expression analysis of three calcium-binding proteins from human heart. Biochemistry 31: 10258-10264, 1992. |
| Uniprot ID | S100A4: P26447 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- S100A4 antibody (A01217-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human A549 whole cell lysates,Lane 2: Human HELA whole cell lysates,Lane 3: Human HELA whole cell lysates,Lane 4: Human THP-1 whole cell lysates.Use rabbit anti- S100A4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for S100A4 at approximately 12KD. The expected band size for S100A4 is at 12KD.|Figure 2.IHC analysis using anti- S100A4 antibody (A01217-2). detected in paraffin-embedded section of Human intestine Cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3.IHC analysis using anti- S100A4 antibody (A01217-2). detected in paraffin-embedded section of Human lung Cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4.IHC analysis using anti- S100A4 antibody (A01217-2). detected in paraffin-embedded section of Human prostatic Cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5.IHC analysis using anti- S100A4 antibody (A01217-2). detected in paraffin-embedded section of Human breast Cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6.ICC analysis using anti- S100A4 antibody (A01217-2). was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue). |Figure 7.Flow cytometry analysis of THP-1 cell (1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-S100A4 Antibody(A01217-2-100ul)
￥1960
