- ￥1180
- BOSTER
- M01891-1-50ul
- 中国
- 2025年07月13日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M01891-1 |
|---|---|
| 产品名称 | Anti-Rab5/RAB5A Antibody (monoclonal, 3E9) |
| 基因名 | RAB5A |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:3E9) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 24KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5 (179-215aa KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN), different from the related mouse and rat sequences by three amino acids. |
| 内容 | 200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 200ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation. |
| Uniprot ID | RAB5A: P20339 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1.ICC analysis using anti-RAB5A antibody (M01891-1) was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue). |Figure 2. Western blot analysis of anti- Rab5/RAB5A antibody (M01891-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human A549 whole cell lysates.
Lane 4: human A375 whole cell lysates,
Lane 5: human SW620 whole cell lysates,
Lane 6: human U20S whole cell lysates,
Lane 7: human HEK293 whole cell lysates,
Lane 8: human U87 whole cell lysates,
Lane 9: rat brain tissue lysates,
Lane 10: mouse brain tissue lysates,
Lane 11: mouse Neuro-2a whole cell lysates.Use rabbit anti- Rab5/RAB5A 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Rab5/RAB5A at approximately 24KD. The expected band size for Rab5/RAB5A is at 24KD.|Figure 3.Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human A549 whole cell lysates.
Lane 4: human A375 whole cell lysates,
Lane 5: human SW620 whole cell lysates,
Lane 6: human U20S whole cell lysates,
Lane 7: human HEK293 whole cell lysates,
Lane 8: human U87 whole cell lysates,
Lane 9: rat brain tissue lysates,
Lane 10: mouse brain tissue lysates,
Lane 11: mouse Neuro-2a whole cell lysates.Use rabbit anti- Rab5/RAB5A 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Rab5/RAB5A at approximately 24KD. The expected band size for Rab5/RAB5A is at 24KD.|Figure 3.Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Rab5/RAB5A Antibody (monoclonal, 3E9)(M01891-1-50ul)
￥1180
