- ￥1180
- BOSTER
- BM0001-50ul
- 中国
- 2025年07月15日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | BM0001 |
|---|---|
| 产品名称 | Anti-ACTA1 Antibody (monoclonal, 5C5) |
| 基因名 | ACTA1 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:5C5) |
| 抗体亚型 | Mouse IgM |
| 分子量 | 42KD |
| 免疫原 | A synthetic peptide |
| 内容 | 200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 200ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. |
| Uniprot ID | ACTA1: P68133 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:100-400 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-ACTA1 antibody (BM0001). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat skeletal muscle tissue lysates,
Lane 2: Mouse skeletal muscle tissue lysates,Use rabbit anti- ACTA1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ACTA1 at approximately 42KD. The expected band size for ACTA1 is at 42KD.|Figure 2.IHC analysis using anti-ACTA1 antibody (BM0001). detected in paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3.IHC analysis using anti-ACTA1 antibody (BM0001). detected in paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4.IHC analysis using anti-ACTA1 antibody (BM0001). detected in paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]
Lane 1: Rat skeletal muscle tissue lysates,
Lane 2: Mouse skeletal muscle tissue lysates,Use rabbit anti- ACTA1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ACTA1 at approximately 42KD. The expected band size for ACTA1 is at 42KD.|Figure 2.IHC analysis using anti-ACTA1 antibody (BM0001). detected in paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3.IHC analysis using anti-ACTA1 antibody (BM0001). detected in paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4.IHC analysis using anti-ACTA1 antibody (BM0001). detected in paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]
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