- ￥1180
- BOSTER
- M00125-3-50ul
- 中国
- 2025年07月01日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M00125-3 |
|---|---|
| 产品名称 | Anti-PCNA Antibody (monoclonal, 2G2) |
| 基因名 | PCNA |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:2G2) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 36KD |
| 免疫原 | E.coli-derived human PCNA recombinant protein (Position: M1-S261). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
| 研究类别 | 1. Moldovan GL, Pfander B, Jentsch S (May 18, 2007). "PCNA, the maestro of the replication fork". Cell. 129 (4): 665–79. 2. Warbrick E (Mar 1998). "PCNA binding through a conserved motif". BioEssays. 20 (3): 195–9. 3. Mailand N, Gibbs-Seymour I, Bekker-Jensen S (May 2013). "Regulation of PCNA-protein interactions for genome stability". Nature Reviews. Molecular Cell Biology. 14 (5): 269–82. |
| Uniprot ID | PCNA: P12004 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- PCNA Antibody (M00125-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hek293 whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: Colo320 whole cell lysates,
Lane 4: K562 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: Rwa264.7 whole cell lysates.
Use mouse anti- PCNA 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PCNA at approximately 36KD. The expected band size for PCNA is at 29KD.|Figure 2. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human clymphadenoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 8. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of Rat colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 9. ICC analysis using anti- PCNA Antibody (M00125-3). was detected in immersion fixed HEP3B cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 10. Flow cytometry analysis of JK cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Hek293 whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: Colo320 whole cell lysates,
Lane 4: K562 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: Rwa264.7 whole cell lysates.
Use mouse anti- PCNA 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PCNA at approximately 36KD. The expected band size for PCNA is at 29KD.|Figure 2. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human clymphadenoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 8. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of Rat colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 9. ICC analysis using anti- PCNA Antibody (M00125-3). was detected in immersion fixed HEP3B cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 10. Flow cytometry analysis of JK cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-PCNA Antibody (monoclonal, 2G2)(M00125-3-50ul)
￥1180
