- ￥1960
- BOSTER
- PB1039-100ul
- 中国
- 2025年07月13日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | PB1039 |
|---|---|
| 产品名称 | Anti-APOE Antibody |
| 基因名 | APOE |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 36KD |
| 免疫原 | E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. APOE ε variants increase risk of warfarin-related intracerebral hemorrhage.Falcone GJ, et al. Neurology, 2014 Sep 23. 2. Calabuig-Navarro MV, et al. Apolipoprotein E genotype has a modest impact on the postprandial plasma response to meals of varying fat composition in healthy men in a randomized controlled trial. J Nutr, 2014 Nov. |
| Uniprot ID | APOE: P08226 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Apolipoprotein E expression in mouse spleen extract (lane 1) and mouse kidney extract (lane 2). Apolipoprotein E at 36KD was detected using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody (Catalog # PB1039) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).|Figure 2. Apolipoprotein E was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody (Catalog # PB1039) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).|Figure 3. Apolipoprotein E was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody (Catalog # PB1039) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).|Figure 4. ICC analysis using anti- APOE antibody (PB1039). was detected in immersion fixed HEPA1-6 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 5. IF analysis of APOE using anti-APOE antibody (PB1039).
APOE was detected in a paraffin-embedded section of mouse liver tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).| Figure 6. IF analysis of APOE using anti-APOE antibody (PB1039).
APOE was detected in a paraffin-embedded section of rat liver tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).[/list_product_images]
APOE was detected in a paraffin-embedded section of mouse liver tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).| Figure 6. IF analysis of APOE using anti-APOE antibody (PB1039).
APOE was detected in a paraffin-embedded section of rat liver tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).[/list_product_images]
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Anti-Apolipoprotein E/APOE Antibody(PB1039-100ul)
￥1960
