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- BOSTER
- A09735-50ul
- 中国
- 2025年07月11日
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- 规格：
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产品概况
| 货号 | A09735 |
|---|---|
| 产品名称 | Anti-ATP5G1,2,3/ATP5MC1,2,3 Antibody |
| 基因名 | ATP5MC1,2,3 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 10-14KD |
| 免疫原 | E.coli-derived human ATP5G1,2,3/ATP5MC1,2,3 recombinant protein (Position: D62-L113). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | The ATP5MC1 gene is one of three human paralogs that encode membrane subunit c of the mitochondrial ATP synthase. It is mapped to 17q21.32. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified. |
| 研究类别 | 1. Dyer, M. R., Walker, J. E. Sequences of members of the human gene family for the c subunit of mitochondrial ATP synthase. Biochem. J. 293: 51-64, 1993. 2. Walker, J. E. Determination of the structures of respiratory enzyme complexes from mammalian mitochondria. Biochim. Biophys. Acta 1271: 221-227, 1995. 3. Yan, W. L., Lerner, T. J., Haines, J. L., Gusella, J. F. Sequence analysis and mapping of a novel human mitochondrial ATP synthase subunit 9 cDNA (ATP5G3). Genomics 24: 375-377, 1994. |
| Uniprot ID | ATP5MC1,2,3: P05496 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:2000-5000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. IF analysis of ATP5G1,2,3 using anti- ATP5G1,2,3 antibody (A09735)
ATP5G1,2,3 was detected in paraffin-embedded section of human intestinal cancer tissuesanti- ATP5G1,2,3 Antibody (A09735) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 2. Flow Cytometry analysis of HL-60 cells using anti- ATP5G1,2,3 antibody (A09735).
Overlay histogram showing HL-60 cells stained with A09735 (Blue line).anti- ATP5G1,2,3 Antibody (A09735,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. Flow Cytometry analysis of PC-3 cells using anti- ATP5G1,2,3 antibody (A09735).
Overlay histogram showing HL-60 cells stained with A09735 (Blue line).anti- ATP5G1,2,3 Antibody (A09735,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 4. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human Ovarian cancer tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human tonsil tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human glioma tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human lung cancer tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 8. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human placenta tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 9. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of mouse intestine tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 10. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of rat intestine tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 11. Western blot analysis of anti- ATP5G1,2,3 antibody (A09735).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human hepG2 whole cell lysates,Lane 2: Human A549 whole cell lysates,Lane 3: Human PC-3 whole cell lysates,Lane 4: Human HELA whole cell lysates,Lane 5: Human HEK293 whole cell lysates,Lane 6: Human U937 whole cell lysates,Lane 7: Human CACO-2 whole cell lysates,Lane 8: rat kidney tissue lysates,Lane 9: rat stomach tissue lysates,Lane 10: rat liver tissue lysates,Lane 11: mouse kidney tissue lysates,Lane 12: mouse stomach tissue lysates,Lane 13: mouse liver tissue lysates,Lane 14: mouse lung tissue lysates,Lane 15: Mouse NEURO-2A whole cell lysates.Use rabbit anti- ATP5G1,2,3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP5G1,2,3 at approximately 14KD. The expected band size for ATP5G1,2,3 is at 14KD.[/list_product_images]
ATP5G1,2,3 was detected in paraffin-embedded section of human intestinal cancer tissuesanti- ATP5G1,2,3 Antibody (A09735) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 2. Flow Cytometry analysis of HL-60 cells using anti- ATP5G1,2,3 antibody (A09735).
Overlay histogram showing HL-60 cells stained with A09735 (Blue line).anti- ATP5G1,2,3 Antibody (A09735,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. Flow Cytometry analysis of PC-3 cells using anti- ATP5G1,2,3 antibody (A09735).
Overlay histogram showing HL-60 cells stained with A09735 (Blue line).anti- ATP5G1,2,3 Antibody (A09735,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 4. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human Ovarian cancer tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human tonsil tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human glioma tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human lung cancer tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 8. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of human placenta tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 9. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of mouse intestine tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 10. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).ATP5G1,2,3 was detected in paraffin-embedded section of rat intestine tissues. anti-ATP5G1,2,3 Antibody (A09735) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 11. Western blot analysis of anti- ATP5G1,2,3 antibody (A09735).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human hepG2 whole cell lysates,Lane 2: Human A549 whole cell lysates,Lane 3: Human PC-3 whole cell lysates,Lane 4: Human HELA whole cell lysates,Lane 5: Human HEK293 whole cell lysates,Lane 6: Human U937 whole cell lysates,Lane 7: Human CACO-2 whole cell lysates,Lane 8: rat kidney tissue lysates,Lane 9: rat stomach tissue lysates,Lane 10: rat liver tissue lysates,Lane 11: mouse kidney tissue lysates,Lane 12: mouse stomach tissue lysates,Lane 13: mouse liver tissue lysates,Lane 14: mouse lung tissue lysates,Lane 15: Mouse NEURO-2A whole cell lysates.Use rabbit anti- ATP5G1,2,3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP5G1,2,3 at approximately 14KD. The expected band size for ATP5G1,2,3 is at 14KD.[/list_product_images]
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Anti-ATP5G1,2,3/ATP5MC1,2,3 Antibody(A09735-50ul)
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