- ￥1180
- BOSTER
- A01911-50ul
- 中国
- 2025年07月05日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01911 |
|---|---|
| 产品名称 | Anti-ITGA5 Antibody |
| 基因名 | ITGA5 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 114KD |
| 免疫原 | E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. |
| 研究类别 | 1. Sosnoski DM, Emanuel BS, Hawkins AL, van Tuinen P, Ledbetter DH, Nussbaum RL, Kaos FT, Schwartz E, Phillips D, Bennett JS, et al. (Aug 1988).2. "Entrez Gene: ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide)". |
| Uniprot ID | ITGA5: P08648 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section IHC | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF) | 1:50-400 |
| Flow cytometry (FCM) | 1-3 μg/1x106 cells |
| Immunofluorescence (IF) | 1:50-400 |
| Direct ELISA | 1:100-100 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (A01911). Lane 1: human Hela cell lysate,Lane 2: human HepG2 cell lysate,Lane 3: rat liver tissue lysate,Lane 4: mouse liver tissue lysate. anti-Integrin alpha 5 antigen affinity purified polyclonal antibody (Catalog # A01911)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for Integrin alpha 5 at approximately 150KD. The expected band size for Integrin alpha 5 is at 114KD.|Figure 2. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (A01911).Integrin alpha 5 was detected in paraffin-embedded section of human colon cancer tissue. anti-Integrin alpha 5 Antibody (A01911) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (A01911).Integrin alpha 5 was detected in paraffin-embedded section of human lung cancer tissue. anti-Integrin alpha 5 Antibody (A01911) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (A01911).Integrin alpha 5 was detected in paraffin-embedded section of mouse cardiac muscle tissue . anti-Integrin alpha 5 Antibody (A01911) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (A01911).Integrin alpha 5 was detected in paraffin-embedded section of rat cardiac muscle tissue . anti-Integrin alpha 5 Antibody (A01911) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (A01911).Integrin alpha 5 was detected in paraffin-embedded section of human placenta tissue . anti-Integrin alpha 5 Antibody (A01911) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. Flow Cytometry analysis of A431 cells using anti- Integrin alpha 5 antibody (A01911).Overlay histogram showing A431 cells stained with A01911 (Blue line).anti- Integrin alpha 5 Antibody (A01911,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 8. Flow Cytometry analysis of HT-1080 cells using anti- Integrin alpha 5 antibody (A01911).Overlay histogram showing HT-1080 cells stained with A01911 (Blue line).anti- Integrin alpha 5 Antibody (A01911,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 9.ICC analysis using anti-Integrin alpha 5 antibody (A01911). was detected in immersion fixed HELA cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue). |Figure 10.IF analysis using anti-Integrin alpha 5 antibody (A01911). detected in paraffin-embedded section of human colon cancer intestine tissue. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue). |Figure 11.IF analysis using anti-Integrin alpha 5 antibody (A01911). detected in paraffin-embedded section of human placenta tissue. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue).[/list_product_images]
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Anti-Integrin alpha 5/ITGA5 Antibody(A01911-50ul)
￥1180
