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- BOSTER
- A04874-1-50ul
- 中国
- 2025年07月07日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A04874-1 |
|---|---|
| 产品名称 | Anti-CTSE Antibody |
| 基因名 | CTSE |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 43KD |
| 免疫原 | E. coli-derived human Cathepsin E recombinant protein (Position: A150-R393). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Cathepsin E is an enzyme that in humans is encoded by the CTSE gene. This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration. |
| 研究类别 | 1. Azuma T, Pals G, Mohandas TK, Couvreur JM, Taggart RT (October 1989). "Human gastric cathepsin E. Predicted sequence, localization to chromosome 1, and sequence homology with other aspartic proteinases". The Journal of Biological Chemistry. 264 (28): 16748–53.2. Couvreur JM, Azuma T, Miller DA, Rocchi M, Mohandas TK, Boudi FA, Taggart RT (Aug 1990). "Assignment of cathepsin E (CTSE) to human chromosome region 1q31 by in situ hybridization and analysis of somatic cell hybrids". Cytogenetics and Cell Genetics. 53 (2-3): 137–9. |
| Uniprot ID | CTSE: P14091 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| ELISA(Cap): | 1:50-1:200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Cathepsin E using anti-Cathepsin E antibody (A04874-1). Lane 1: rat stomach tissue lysates, Lane 2: mouse stomach tissue lysates, Lane 3: mouse SP20 whole cell lysates. anti-Cathepsin E antigen affinity purified polyclonal antibody (Catalog # A04874-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for Cathepsin E at approximately 48KD. The expected band size for Cathepsin E is at 43KD.|Figure 2. IHC analysis of Cathepsin E using anti-Cathepsin E antibody (A04874-1).Cathepsin E was detected in paraffin-embedded section of human Lung cancer tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of Cathepsin E using anti-Cathepsin E antibody (A04874-1).Cathepsin E was detected in paraffin-embedded section of human gastric cancer tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. [/list_product_images]
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Anti-Cathepsin E/CTSE Antibody(A04874-1-50ul)
￥1180
