- ￥1960
- BOSTER
- A02632-1-100ul
- 中国
- 2025年07月12日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A02632-1 |
|---|---|
| 产品名称 | Anti-CDH5 Antibody |
| 基因名 | CDH5 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 88KD/130KD |
| 免疫原 | E. coli-derived human VE Cadherin recombinant protein (Position: D48-R272). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels. |
| 研究类别 | 1. Huber, P., Dalmon, J., Engiles, J., Breviario, F., Gory, S., Siracusa, L. D., Buchberg, A. M., Dejana, E. Genomic structure and chromosomal mapping of the mouse VE-cadherin gene (Cdh5). Genomics 32: 21-28, 1996.2. Kremmidiotis, G., Baker, E., Crawford, J., Eyre, H. J., Nahmias, J., Callen, D. F. Localization of human cadherin genes to chromosome regions exhibiting cancer-related loss of heterozygosity. Genomics 49: 467-471, 1998. |
| Uniprot ID | CDH5: P33151 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in frozen section (IHC-F): | 1:50-400 |
| (ELISA)： | 1:100-1000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-CDH5 antibody (A02632-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates. Use rabbit anti-CDH5 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CDH5 at approximately 130KD. The expected band size for CDH5 is at 130KD.|Figure 2. Immunohistochemical analysis of paraffin embedding human Lung cancer tissue slice at a dilution of 1:500.|Figure 3.Immunohistochemical analysis offrozen section human placenta at a dilution of 1:50 dylight 488 goat anti-rabbit IgG(green) blue Pseudocolor blue=DAPI.|Figure 4.Immunofluorescence assay analysis of A549 cell at a dilution of 1:50 dylight 488 goat anti-rabbit IgG(green) blue Pseudocolor blue=DAPI.|Figure 5.Flow cytometry analysis of A431 cell(1x106) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).[/list_product_images]
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Anti-VE Cadherin/CDH5 Antibody(A02632-1-100ul)
￥1960
