- ￥500 - 3999
- FutureBio
- FBKTC000045Y
- 美国
- 2025年09月01日
- ELISA (Verified)
- Rabbit
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
CTSE
- 应用范围：
ELISA (Verified)
- 宿主：
Rabbit
- 适应物种：
Mouse
- 标记物：
Unconjugated
- 克隆性：
monoclonal
- 保存条件：
stored at 2℃-8℃ for one month, stored at -20℃ to -80℃ for one year.
- 形态：
See the lable
- 亚型：
liquid
- 免疫原：
IgG
- 规格：
10/50/100/200 ul
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
throughout the procedure. d) Plate the entire fusion out in just 120 x 1ml cultures in 6 x 'Linbro' 24 well plates, using DMEM + 20% FCS (containing penniclin + streptomicin, Na pyruvate and glutamine). e) DO NOT use Isoves medium (IMDM) as this will encourage
T-Cell Activation Using mAb to CD3
µg/ml solution of anti-CD3e (145-2C11) in sterile PBS. Calculate the number of wells required for each experimental condition and consider triplicate samples for each condition. For example, to coat one-half plate (48 wells) 2.6ml of antibody solution
step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
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