Morunigrol C,1174134-94-1

Single Primer ("Semi-Random") P

to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI

Single tube confirmation PCR protocol

until the PCR is started. Alternatively, a "hot start" can be performed by adding the Taq polymerase to the individual tubes after the PCR mixtures have been heated to 94°C. Hot start improves the PCR results but this step is labor intensive

Worm PCR

onto the top of the mineral oil overlay. Set sample in ice bucket until all samples have been prepared. Microfuge briefly to move the master mix through the mineral oil overlay. Rapidly heat the samples to 94°C and cycle 30 times through a program appropriate