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文献和实验Generation of Recombinant Adenovirus Using the Escherichia coli BJ5183 Recombination System
here uses homologous recombination machinery of Escherichia coli BJ5183 to construct plasmids used in generation of adenoviral vectors. With this method, no ligation steps are involved in generating the plasmids, and any region of the adenoviral genome
Screening for the Expression of Soluble Recombinant Protein in Escherichia coli
Protein expression and purification have traditionally been time-consuming, case-specific endeavors, and are considered to be the greatest bottlenecks in most proteomics pipelines. Escherichia coli (E. coli ) is the most convenient and cost
Automated Recombinant Protein Expression Screening in Escherichia coli
, Strasbourg for performing recombinant protein expression screening in Escherichia coli . The procedure consists of parallel competent cells transformation, cell plating, and liquid culture inoculation, implemented for up to 96 samples at a time.
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