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文献和实验Expression of Recombinant Proteins with Uniform N-Termini
that recombinant proteins produced in this expression system contain homogeneous, unprocessed N-termini.
High Throughput Production of Recombinant Human Proteins for Crystallography
This chapter presents in detail the process used in high throughput bacterial production of recombinant human proteins for crystal structure determination. The core principles are: (1 ) Generating at least 10 truncated constructs
and centrally located DNA-binding domain (DBD), and a poorly characterized N-terminal domain that is required for maximal transcriptional activity (1 ,2 ) Human PR is expressed as two proteins from a single gene by alternate use of two promoters: PR
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