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文献和实验In Vitro Transcription and Translation in a Cell-Free System from Clostridium tetani
or degradation of short-lived mRNA (5 , 6 ). The combined transcription/translation system from E. coli did not give satisfactory results with tetanus toxin (TeTx)-specific DNA (Fig. 1 ). Translation of in vitro transcribed TeTx-specific mRNA in reticulocyte
Genetic Detoxification of Bacterial Toxins
Several pathogens, such as Corynebacterium diphtheriae, Clostridium tetani, Bordetella pertussis, Vibrio cholerae , enterotoxigenic Escherichia co1i (1 ), and even some emerging pathogens, such as Helicobacter pylori (2 ), produce potent
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botulinum neurotoxin serotype FI, 240 mg/kg cells307Clostridium botulinum neurotoxin serotype A binding domainI, 2.4 mg total[59]Clostridium tetani Tetanus toxin fragment CI, 12 g/L[60]Diphtheria anti-human anti-T cell immunotoxinS, 10 mg/L, α-MF
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