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- 2025年07月13日
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文献和实验Detection of Recombinant Viral Coat Protein in Transgenic Plants
The discovery that the expression of a viral coat protein in transgenic plants confers protection to infection by homologous and related viruses (1 –4 ) revolutionized the field of plant breeding. The approach of “coat protein-mediated
High Throughput Production of Recombinant Human Proteins for Crystallography
This chapter presents in detail the process used in high throughput bacterial production of recombinant human proteins for crystal structure determination. The core principles are: (1 ) Generating at least 10 truncated constructs
A Novel Method for the Production of Fully Modified K-Ras 4B
post-translationally modified proteins in bacterial expression systems is often problematic. Here, we describe a novel recombinant approach to prepare human K-Ras 4B, a protein that is post-translationally farnesylated
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