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- 2025年07月10日
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文献和实验Assay for Protein Modification by Poly-ADP-Ribose In Vitro
, the resulting modified proteins have unpredictable final masses, making it difficult to �identify acceptor amino acids. Using recombinant proteins, in vitro pADP ribosylation assay and mass spectrometry, we have been able to identify conserved Glu residue
Introduction of human 14-3-3 proteins
protein kinase II,regulation of protein kinase C,phospholipase A2 activity,stimulation of exocytosis and activation of bacterial exoenzyme S (ExoS)during ADP-ribosylation of host proteins.In addition,a plant 14-3-3 protein is present in a G-box DNA/protein
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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