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文献和实验H. pylori DNA Transformation by Natural Competence and Electroporation
Helicobacter pylori is an important etiological pathogen of human stomach diseases, such as gastritis, peptic ulcer, and gastric carcinoma (1 ). In the past few years, great progress has been made in the cloning and characterization of H
In this chapter we describe efficient procedures for the construction, expression and screening of comprehensive libraries of human Fab antibody fragments displayed on the surface of filamentous phage. Phagemid vectors are used for placing
Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
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