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- PeproTech
- AF-300-27-200UG
- 2025年07月10日
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- 规格：
200ug
Animal-Free Human Leptin (200ug)
|
Product Details |
|
|
Size |
200 μg |
|
Species |
Human |
|
Expression system |
E. coli |
|
Amino acid sequence |
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC |
|
Molecular weight |
16 kDa |
|
Class |
Recombinant |
|
Type |
Protein |
|
Purity |
98% by SDS-PAGE gel and HPLC analyses. |
|
Endotoxin concentration |
<0.1 EU/µg |
|
Conjugate |
Unconjugated |
|
Form |
Lyophilized |
|
Purification |
purified |
|
Contains |
no preservative |
|
Storage conditions |
-20°C |
|
|
|
|
Applications Tested Dilution Publications |
|
|
Functional Assay (Functional) Assay-dependent - |
|
Product Specific Information
Recombinant Human Leptin is a 16.0 kDa protein containing 147 amino acid residues.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis
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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions Sohyun L. McElroy1 and Renee A. Reijo Pera Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine
RNA Purification Protocol for 100-200 mg of Animal Tissue
. Storage at -70°C. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 100-200 mg frozen ground tissue or fresh tissue to a 15 mL centrifuge tube containing 6 ml RCL Buffer. Homogenize the sample using 5-10
Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions
and future clinical application require an animal-free and well-defined culture system for hESCs, these conventional conditions would prevent the use of hESCs in human therapy. This chapter describes optional culture conditions based on either animal-free
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