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PeproTech Animal-Free Murine I

L-21 (250ug)
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  • ¥25715
  • PeproTech已认证
  • AF-210-21-250UG
  • 2025年07月13日
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    • 文献和实验
    • 技术资料
    • 规格

      250ug

    Animal-Free Murine IL-21 (250ug) 

    IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation.
    Its action generally enhances antigen-specific responses of immune cells. The biological effects
    of IL-21 include: inducing the differentiation of T-cell-stimulated B-cells into plasma cells and
    memory B-cells; the stimulation of IgG production in conjunction with IL-4; and the induction
    of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling.
    Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts
    its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the g
    chain (gc) found in other cytokine receptors, including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/
    IL-21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases
    JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.

    Product Details

    Size

    250 µg

    Species

    Mouse

    Published Species

    Mouse

    Expression system

    E. coli

    Amino acid sequence

    MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK  LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS

    Molecular weight

    15 kDa

    Class

    Recombinant

    Type

    Protein

    Purity

    98% by SDS-PAGE gel and HPLC analyses.

    Endotoxin concentration

    <0.1 EU/µg

    Activity

    Determined by its ability to stimulate the proliferation of human ANBL-6 cells. The expected ED50 is 1.0 ng/ml, corresponding to a specific activity of  1 x 10^6 units/mg.

    Conjugate

    Unconjugated

    Form

    Lyophilized

    Purification

    purified

    Contains

    no preservative

    Storage conditions

    -20°C

     

    Applications

    Tested Dilution

    Publications

    Functional Assay (Functional)

    Assay-dependent

    -

    In vitro Assay (IV)

    -

    2 Publications

     

     

    Product Specific Information

    Recombinant Murine IL-21 is a 15.0 kDa protein consisting of 130 amino acid residues.

    This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis

     

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    图标文献和实验
    该产品被引用文献

    [1]Ricker E, Manni M, Flores-Castro D, Jenkins D, Gupta S, Rivera-Correa J, Meng W, Rosenfeld AM, Pannellini T, Bachu M, Chinenov Y, Sculco PK, Jessberger R, Prak ETL, Pernis AB. Altered function and differentiation of age-associated B cells contribute to the female bias in lupus mice. Nat Commun. 2021 Aug 10;12(1):4813. doi: 10.1038/s41467-021-25102-8. PMID: 34376664; PMCID: PMC8355159.

    [2]Takahara T, Matsuo K, Seto M, Nakamura S, Tsuzuki S. Synergistic activity of Card11 mutant and Bcl6 in the development of diffuse large B-cell lymphoma in a mouse model. Cancer Sci. 2016 Nov;107(11):1572-1580. doi: 10.1111/cas.13057. Epub 2016 Nov 4. PMID: 27560392; PMCID: PMC5132338.

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    文献支持
    PeproTech Animal-Free Murine IL-21 (250ug)
    ¥25715