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- 文献和实验
- 技术资料
- 克隆性:
PK136
- 标记物:
PerCP-Cy5.5
- 规格:
25 μg
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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
Mouse Th1/Th2 细胞群检测 CD3-PerCP-Cy5.5 PCP55-30002 CD4-FITC FITC-30128 IFN-γ-PE PE-30074 IL4-APC APC-30026 Human Treg细胞群检测 CD4-FITC FITC-30005 CD25-PE PE-30035 CD3-PerCP-Cy5.5 PCP55-30004 CD127-FineTest®647
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