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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
XMG1.2
- 标记物:
BG Violet 450
- 规格:
25 μg
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文献和实验tumor necrosis factor–{alpha} (TNF-{alpha}), interferon-beta (IFN-beta), and IFN-{gamma} (1). The authors observed that of ~200 miRNAs examined, only miR-155 increased in amount in response to both TLR ligands and IFN-beta. miR-155 accumulated
,表现出Th1、Th2和TFH等具有传统功能的细胞亚群。导致Foxp3下调的关键因素包括高水平的炎症因子内环境,如可诱导效应性T细胞产生的细胞因子:IL-6和IFN-gamma。Tregs 细胞中Foxp3的表达分析CD4+CD25+ Treg 细胞中Foxp3 +细胞群表明与Foxp3+Treg完全重合。使用Foxp3鉴定Treg用Anti-Mouse CD4 FITC、Anti-Mouse CD25 APC和Anti-Mouse/Rat Foxp3 PE 标记BALB/c小鼠 脾细胞. 右上
Intracellular Cytokine Staining Protocol
by flow cytometry since cytokine levels are typically too low in freshly-prepared cells. Stimulation of cells with the appropriate reagent will depend on the cell type and the experimental conditions. For example, to stimulate T cells to produce IFN-gamma
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